DIRECTIONS: Using letters A, B, and C, arrange the stages/steps on how the different techniques/tools in genetic engineering work., 1. MAKING A RECOMBINANT DNA, _____ Sticky ends are joined by DNA ligase, _____ Restriction enzymes cut the desired DNA sequence, _____ Study and determine the specific gene needed , 2. POLYMERASE CHAIN REACTION / MAKING DNA COPIES , _____ DNA polymerase starts making copies , _____ 201cprimers201d provide a lace for DNA polymerase, _____ DNA is heated to separate the strands and cooled to bind , 3. TRANSFORMING BACTERIA , _____ Insert a human gene into plasmid / bacterial DNA , _____ The recombinant DNA is inserted in the bacterial cell , _____ The bacteria can produce the human protein , 4. TRANSFORMING PLANT CELLS _____ The plasmid can be used to infect plant cells that take up DNA on its own , _____ Insert a piece of foreign DNA into the plasmid , _____ Inactivate the tumor-producing gene of a bacterium , 5. TRANSFORMING ANIMAL CELLS _____ The host gene may lose (knocked out) sequences in between , _____ DNA molecule is constructed with two ends that may recombine with specific sequences , _____ The knocked-out gene is replaced with a new gene